Abstract：Objective To investigate the torsional mechanical properties of New Zealand rabbit radius at different months of age and to provide biomechanical reference for the preparation of rabbit radius bone defect model and the evaluation of bone tissue engineering materials.Methods Fifteen male New Zealand rabbits were divided into 3 groups according to months after birth: 3-month group, 6-month group and 9-month group, five rabbits in each group. The experimental rabbits were killed by implementing air embolism. Both sides of the radius were taken, totally 10 bones in each group. The specimens were placed in the mold and filled, embedded and fixed with polymethyl methacrylate. Both the ends of the bone specimens were placed in the clamp of the torsion tester. Torque was applied to the specimens at a speed of 5°/min until they were broken. The maximum torque and the maximum torsional angle were obtained, and the maximum shear stress of torsion was calculated. At the same time, the fracture morphology of the radius was observed.Results The three biomechanical indexes, the maximum torque, the maximum torsional angle and the maximum shear stress in rabbit radius were (292.23±41.71) Nmm, 18.93°±2.67° and (10.67±0.79) MPa in the 3-month group; (741.92 ±66.65) Nmm, 11.95°±4.02° and (17.85±1.33) MPa in the 6-month group; (747.77 ±65.96)Nmm, 10.19°±3.16° and (18.10±1.25) MPa in the 9-month group, respectively. The maximum torque and the maximum shear stress in the 3-month group were much lower than those in the 6-month group and 9-month group. The maximum torsional angle was larger than those of the 6-month group and the 9-month group. The differences were statistically significant (all P values＜0.01). However, there were no significant differences of the above-mentioned three indexes between the 6-month group and the 9-month group (all P values＞0.05). The fractures of the 5 radii in the 3-month group were spiral, of which one radius only showed cracks and the rest 4 radii showed greenstick fractures. The radii in the 6-month group and the 9-month group showed spiral fractures.Conclusions There are differences in the torsional mechanical properties of the radius of New Zealand rabbits at different months of age. When the age of rabbit increases to 6 month later, the torsional mechanical properties of the radius show a tendency to be more stable.
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2018, Vol. 23 
