Abstract:Objective To explore the efficiency of micro RNA(miRNA) activity analysis method in screening the miRNA associated with castration resistance in prostate cancer.Methods miRNAs were screened for their potential role in castration resistance of prostate cancer by miRNAs activity analysis method. Human hormone-sensitive prostate cancer LNCAP cells (control group) and castration-resistant prostate cancer C4-2 cells (C4-2 group), PC-3 cells (PC-3 group) and DU-145 cells (DU-145 group) were cultured. Total RNA was extracted from each group. Real-time fluorescence quantitative PCR (qPCR) was used to detect miRNAs and compare the expression of miRNAs in each group.Results According to the screening process, 9 differentially expressed miRNAs were identified by miRNA activity analysis method. They were miR-1, miR-122, miR-218, miR-145, miR-155, miR-210, miR-197, miR-346 and let-7b. The 9 miRNAs were detected by qPCR. The results showed that 7 miRNAs were differentially expressed in prostate cancer cells in two different states. In different castration-resistant prostate cancer cells, miR-210, miR-197, miR-346 and miR-218 were significantly over expressed, while miR-122, miR-145 and let-7b were significantly under expressed.Conclusions The screening of castration-resistant transformation-related miRNAs in prostate cancer by miRNAs activity analysis method has high accuracy and reliability, and the specific transformation process needs further confirmation.
王苏贵, 吴自余, 李强, 李乾, 王磊, 张文宇, 阳东荣, 姜福金. 微小RNA活性分析法筛选前列腺癌去势抵抗转化相关微小RNA的实验研究[J]. 中华解剖与临床杂志, 2018, 23(6): 538-542.
Wang Sugui, Wu Ziyu, Li Qiang, Li Qian, Wang Lei, Zhang Wenyu, Yang Dongrong, Jiang Fujin.. Screening key micro RNAs for castration-resistant prostate cancer based on micro RNA activity analysis method. Chinese Journal of Anatomy and Clinics, 2018, 23(6): 538-542.
Zhang W, Zang J, Jing X, et al. Identification of candidate miRNA biomarkers from miRNA regulatory network with application to prostate cancer[J]. J Transl Med, 2014, 12: 66. DOI:10.1186/1479-5876-12-66
[5]
Wang Y, Rekaya R. LSOSS: detection of cancer outlier differential gene expression[J]. Biomark Insights, 2010, 5: 69-78
[6]
He JH, Han ZP, Zhou JB, et al. MiR-145 affected the circular RNA expression in prostate cancer LNCaP cells[J]. J Cell Biochem, 2018, 119(11): 9168-9177. DOI:10.1002/jcb.27181
Zhou B, Wang J, Zheng G, et al. Methylated urolithin A, the modified ellagitannin-derived metabolite, suppresses cell viability of DU145 human prostate cancer cells via targeting miR-21[J]. Food Chem Toxicol, 2016, 97: 375-384. DOI: 10.1016/j.fct.2016.10.005
[10]
Chen X, Yang C, Xie S, et al. Long non-coding RNA GAS5 and ZFAS1 are prognostic markers involved in translation targeted by miR-940 in prostate cancer[J]. Oncotarget, 2018, 9(1): 1048-1062. DOI:10.18632/oncotarget.23254
[11]
Wu G, Wang J, Chen G, et al. microRNA-204 modulates chemosensitivity and apoptosis of prostate cancer cells by targeting zinc-finger E-box-binding homeobox 1 (ZEB1)[J]. Am J Transl Res, 2017, 9(8): 3599-3610
[12]
Sikand K, Slaibi JE, Singh R, et al. miR 488* inhibits androgen receptor expression in prostate carcinoma cells[J]. Int J Cancer, 2011, 129(4): 810-819. DOI:10.1002/ijc.25753
Robinson JM. MicroRNA expression during demosponge dissociation, reaggregation, and differentiation and a evolutionarily conserved demosponge miRNA expression profile[J]. Dev Genes Evol, 2015, 225(6): 341-351. DOI:10.1007/s00427-015-0520-5
Kojima S, Mulholland DJ, Ettinger S, et al. Differential regulation of IGFBP-3 by the androgen receptor in the lineage-related androgen-dependent LNCaP and androgen-independent C4-2 prostate cancer models[J]. Prostate, 2006, 66(9): 971-986. DOI:10.1002/pros.20420