Abstract：Objective A study was conducted to investigate the pathological changes of abdominal muscle and diaphragm in rabbits with different durations of abdominal hypertension. Methods Twenty-two healthy adult New Zealand rabbits with a body weights in the range of 2.7-3.2 kg were selected. These experimental rabbits were randomly divided into the control group (four rabbits) and model group 1-3 (each group of six rabbits). The animal models of abdominal hypertension were made from the four groups of experimental rabbits. The pressurized water sac was not injected in the control group. In model groups 1-3, normal saline containing gentian violet solution was injected into the pressurized water bag. The intraperitoneal pressure was measured once every 50 mL of normal saline, and finally, the intraperitoneal pressure reached 20 mmHg (1 mmHg=0.133 kPa). In the control group, samples were collected 24 h after the preparation of the model. In model groups 1-3, samples were collected 24, 48, and 72 h after the preparation of the model. The experimental animals were routinely killed by air injection at the planned time point. Complete resection of the left diaphragm and 6 cm × 4 cm anterior abdominal wall tissue of experimental rabbits were prepared from the anterior abdominal wall of experimental rabbits. HE staining was performed. The pathological changes of specimens in each group were observed under a biological light microscope. Results Twenty-two experimental rabbits successfully completed the model preparation. When the intraperitoneal pressure of rabbits in model groups 1-3 reached 20 mmHg, the amount of water in the intraperitoneal pressurized water sac was 260-380 mL. The activity of all experimental animals decreased significantly after preparing the model. The food intake of rabbits in the control group decreased slightly, and the food intake of rabbits in model groups 1-3 decreased significantly, or they did not eat. During the experiment, one rabbit in model groups 1-3 died. (1) The histopathological changes of the abdominal wall tissue were as follows. Fatty degeneration of abdominal wall striated muscle was observed in the control group. In model group 1, many inflammatory cells were observed between abdominal muscles. Bleeding and local inflammatory necrosis were observed between abdominal muscles in model group 2. Striated muscle ruptured with steatosis, and vitreous degeneration/necrosis were observed in model group 3. (2) The pathological changes of diaphragm were as follows. In the control group, partial atrophy of diaphragm striated muscle bundle was observed. In model group 1, partial atrophy and partial hypertrophy of diaphragm and striated muscle bundles were observed. In model group 2, the fasciculus of diaphragm and striated muscle atrophied, the atrophic nuclei gathered, and the striations decreased or disappeared in some areas. In model group 3, partial hypertrophy and atrophy of diaphragm and striated muscle bundles, as well as dilation and congestion of blood vessels between muscle bundles were observed. Conclusion High abdominal hypertension can cause obvious cytopa thological damage to the abdominal muscle and diaphragm, which are gradually aggravated with the extension of the duration of high abdominal hypertension.
王宏业, 杨秀峰, 齐凤龙, 乔进余, 张盼盼, 齐彩霞. 兔腹腔高压对其腹肌、膈肌损伤的病理观察[J]. 中华解剖与临床杂志, 2022, 27(9): 651-655.
Wang Hongye, Yang Xiufeng, Qi Fenglong, Qiao Jinyu, Zhang Panpan, Qi Caixia. Pathologic observation of abdominal muscle and diaphragm injury induced by peritoneal high pressure in rabbits. Chinese Journal of Anatomy and Clinics, 2022, 27(9): 651-655.
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2022, Vol. 27 
