Abstract：Objective To investigate effect on intestinal ischemia-reperfusion injury of mice injected with LP17.Methods 36 mice were divided into three groups randomly(n=9 in each group):Sham operation group(S group), intestinal ischemia-reperfusion group (I/R group), intestinal ischemia-reperfusion+LP17 control peptide group (C1 group), intestinal ischemia-reperfusion+LP17 treatment group (C2 group). Twenty min superior mesenteric artery occlusion in mice produced intestinal ischemia-reperfusion model, 5 min before reperfusion mice by intraperitoneal injection of the drug, 24 h after the mice were sacrificed and serum concentrations of triggering receptor expressed on myeloid cells(sTREM-1) and tumor necrosis factor-α(TNF-α) were observed mucosal the extent of damage, immune staining of liver cell expression of nuclear factor-κB(NF-κB).Results The concentration of serum sTREM-1,the concentration of serum TNF-α and staining integral of the NF-κB shown on liver cell in I/R group(1 686.34±55.98)pg/ml,(121.81±8.01)pg/ml and (3.36±0.62)and C1 group (1 643.73±65.45)pg/m, (119.88±8.05)pg/m, and 3.21±0.94 were the highest and significantly reduced in the C2 group (944.58±39.75)pg/ml,(65.92±4.91)pg/ml,and 0.92±0.55,compared with I/R group and C1 group, it was statistically significant(all P values＜0.01). The small intestine mucosa of the mice in S group was normal under light microscope. The villi in I/R group and C1 group were damaged seriously, villus necrosis significantly; those in C2 group received less damage and the difference had statically significance(all P values＜0.01).Conclusions LP17 can reduce inflammatory response caused by over activation of TNF-α and NF-κB, and reduce the damage of the intestinal mucosa and the distant organ caused by intestinal ischemia-reperfusion injury through regulating the signaling pathway of TREM-1.
孙学童，季涛，张宗兵，孔令尚，刘牧林，何先弟. LP17多肽对小鼠肠缺血再灌注损伤影响[J]. 中华解剖与临床杂志, 2014, 19(4): 320-323.
Sun Xuetong , Ji Tao, Zhang Zongbing, Kong Lingshang, Liu Mulin, He Xiandi.. Effect of LP17 on intestinal ischemia-reperfusion injury of mice. Chinese Journal of Anatomy and Clinics, 2014, 19(4): 320-323.
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