Abstract：Objective To establish intestinal ischemia reperfusion injury model in mice and investigate the changes of triggering recepter expressed on myeloid cell-1(TREM-1) expression in ileum after intestine ischemia reperfusion injury and the relationship between TREM-1 expression and TNF-α level in peripheral blood. Methods Seventy-two mice were randomly divided into 3 groups: normal control group(group N), sham operation group(group S), ischemia reperfusion injury group(group I/R). Intestinal ischemia reperfusion injury model was established, mice in group S and group I/R were respectively sacrificed after 6 h,12 h,24 h and 48 h, enzyme linked immunosorbent assay was used to detect the TREM-1 expression and TNF-α levels in peripheral blood and the correlation between TREM-1 expression and TNF-α levels was analyzed. The expression of TREM-1 in the small intestine tissue was observed by immunohistochemistry. Results The concentration of sTREM-1 in peripheral blood by ELISA in the group R/I reached the peak after 24 h and in this time point the concentration of sTREM-1 in peripheral blood in the group R/I was higher than the groups [(178.58±10.98)pg/ml], the concentration in the group R/I displayed at all time points was higher compared with the group N [(168.99±22.79)pg/ml].The difference was significant (all P values<0.01). TNF-α level in the group R/I began to increase after 12 h[(33.03±4.12)pg/ml] and reached the peak after 24 h[(94.01±9.44)pg/ml], the changes of sTREM-1 expression were positively correlated with the TNF-α levels(r=0.840,P=0.000). Immunohistochemistry showed that the TREM-1 expression in the intestine in mice increased after ischemia reperfusion injury and the staining points was the highest in group I/R 24 h(3.38±0.66) and the positive parts were mainly small intestinal lamina propria and epithelial cells. Conclusions Upregulation of TREM-1 expression in intestinal tissue might lead to dysfunction of intestinal mucosal barrier and it might be an important link to the systemic inflammation. sTREM-1 could be used to determine the severity of intestinal mucosa injury in intestinal ischemia reperfusion injury.
季涛, 孔令尚, 孙学童, 张宗兵, 王栓虎, 汪华学, 刘牧林. 肠缺血再灌注损伤小鼠髓样细胞触发受体-1表达的改变及意义[J]. 中华解剖与临床杂志, 2014, 19(2): 154-158.
Ji Tao, Kong Lingshang, Sun Xuetong, Zhang Zongbing, Wang Shuanhu, Wang Huaxue, Liu Mulin. Change and significance of triggering recepter expressed on myeloid cell-1 expression in mice with intestinal ischemia reperfusion injury. Chinese Journal of Anatomy and Clinics, 2014, 19(2): 154-158.
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2014, Vol. 19 
