Abstract：Objective To investigate the repair mechanism of hepatocyte growth factor (HGF) on intestinal mucosal barrier dysfunction of the severe acute pancreatitis (SAP) mice, and to evaluate the effect of intestinal stem cell on the repair mechanism of intestinal mucosal barrier dysfunction.Methods All healthy male Kunming mice were divided into normal control (n=10), SAP (n=25) and HGF group (n=25) by random location. Serum D-lactate was deteted by automatic biochemistry analyzer and ultraviolet spectrophomotometry. The tissues of mesenteric lymph nodes, pancreas, liver, spleen and lung were cultured to determined baceterial tranlocation. Intestinal mucosal epithelial cells proliferation index(PI)was analyzed by flow cytometry (FCM). The changes of intestinal stem cells in the crypt were observed by immunohistochemistry.Results Compared to NC group (4.87±0.73) mg/L and HGF group (7.30±0.88) mg/L, serum D-lactate was significantly increased in SAP group(11.17±1.90) mg/L(F=78.20, all P values<0.01). In comparison with NC group (14%, 7/50), organ bacterial translocation in tissues of mesteric lymph nodes, pancreas, liver, spleen and lung were significantly increased in SAP group(50%, 40/80, χ2=7.427, P＜0.01),but was lower in HGF group (29%, 29/100) than those in SAP group(50%, 40/80, χ2=4.112, P＜0.01). Compared to NC group,PI wassignificantly decreased in SAP group(P<0.01), but was higher in HGF group than those in SAP group, and was higher in HGF group than those in NC group. Compared to NC group (2.16±0.90), intestinal stem cell was significantly decreased in SAP(1.26±0.87), and was higher in HGF group (2.50±0.96) than those in SAP (F=8.34, all P values<0.05).Conclusions HGF can not only significantly reduce the injury of intestinal mucosa in mice with acute pancreatitis but can also promote the proliferation of intestinal mucosal epithelial cells. Intestinal mucosal permeability of SAP mice and organ bacterial translocation are degraded significantly after using HGF.
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