Effect of LINC01352 on oral squamous cell carcinoma and the underlying mechanism
Hu Mengtian1, Pan Shukuang1, Wang Yuxin2, Xue Hongbao3
1Department of Stomatology, Bengbu Third People's Hospital, Bengbu 233000, China; 2Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital Medical School of Nanjing University, Nanjing 210003, China; 3Public Basic School, Bengbu Medical College, Bengbu 233030, China
Abstract：Objective This study aimed to explore the effect and mechanism of LINC01352 on the proliferation, migration, and invasion of oral squamous cell carcinoma (OSCC). Methods SCC25 cells of OSCC were cultured and divided into LINC01352 overexpression (pc-LINC01352) group, its negative control (pc-NC) group, miR-629-5p mimic group, and its negative control (miR-NC) group. pc-LINC01352, pc-NC, miR-629-5p mimics, and miR-NC were transfected into SCC25 cells in corresponding groups, respectively. After transfection of pc-LINC01352 and pc-NC in SCC25 cells, the relative expression of LINC01352 and miR-629-5p was detected by real-time fluorescence quantitative polymerase chain reaction. Cell viability was detected with a cell counting kit-8 (CCK-8), and cell-proliferation ability was detected by EdU. Cell migration and invasion ability were detected by Transwell assay. The potential downstream target of LINC01352 and the binding sites of LINC01352 was predicted by LncRNASNP2. After transfection of miR-629-5p mimics and miR-NC in SCC25 cells, the potential downstream target genes and binding sites of LINC01352 were verified by dual-luciferase reporter assay. Results After transfection with pc-LINC01352 in SCC25 cells, the relative expression of LINC01352 in the pc-LINC01352 group (4.99±0.77) was higher than that in the pc-NC group (1.00±0.07), and the relative expression of miR-629-5p in the miR-629-5p mimic group (0.32±0.01) was lower than that in the miR-NC group (1.00±0.06); the difference was statistically significant (t=8.94, 15.72, all P values<0.05). CCK-8 results showed that the optical density values of the pc-LINC01352 group at 24, 48 and 72 h were significantly lower than those of the pc-NC group (all P values <0.001). EdU results showed that the positive rate of SCC25 cells in the pc-LINC01352 group were 9.37%±1.30%, which were lower than the 25.92%±2.18% of the pc-NC group, and the difference was statistically significant (t=11.29, P=0.001). The numbers of migrating and invading cells in the pc-LINC01352 group were 63±6 and 50±4, respectively, which were both lower than those in the pc-NC group (186±9 and 146±8, respectively), and the differences were statistically significant (t=20.25, 19.64, all P values <0.05). The potential downstream target gene of LINC01352 was predicted to be miR-629-5p, and the binding site between LINC01352 and miR-629-5p was the 3′ UTR region of miR-629-5p. Results of dual-luciferase reporter assay showed that the luciferase activity of LINC01352-WT in the miR-629-5p mimic group was 0.42±0.04, which was lower than the 1.00±0.05 of the miR-NC group, and the difference was statistically significant (t=19.45, P<0.001). Meanwhile, no significant difference in the luciferase activity of LINC01352-MT was found between the two groups (P>0.05). Conclusion The upregulation of LINC01352 expression in OSCC cell line SCC25 cells can decrease the cell viability, proliferation, migration, and invasion of SCC25 cells. The mechanism may involve the direct binding of LLINC01352 to the 3′ UTR region of miR-629-5p and the subsequent downregulation of miR-629-5p expression.
胡梦甜, 潘树矿, 王育新, 薛洪宝. LINC01352对口腔鳞状细胞癌的影响及其作用机制[J]. 中华解剖与临床杂志, 2023, 28(12): 819-825.
Hu Mengtian, Pan Shukuang, Wang Yuxin, Xue Hongbao. Effect of LINC01352 on oral squamous cell carcinoma and the underlying mechanism. Chinese Journal of Anatomy and Clinics, 2023, 28(12): 819-825.
Sung H, Ferlay J, Siegel RL, et al.Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021,71(3):209-249. DOI: 10.3322/caac.21660
[2]
Melo-Alvim C, Neves ME, Santos JL, et al.Radiotherapy, chemotherapy and immunotherapy-current practice and future perspectives for recurrent/metastatic oral cavity squamous cell carcinoma[J]. Diagnostics (Basel), 2022,13(1):99. DOI: 10.3390/diagnostics13010099
[3]
Dan H, Liu S, Liu J, et al.RACK1 promotes cancer progression by increasing the M2/M1 macrophage ratio via the NF-κB pathway in oral squamous cell carcinoma[J]. Mol Oncol, 2020,14(4):795-807. DOI: 10.1002/1878-0261.12644
[4]
Roi A, Roi CI, Negruțiu ML, et al.The challenges of OSCC diagnosis: salivary cytokines as potential biomarkers[J]. J Clin Med, 2020,9(9):2866. DOI: 10.3390/jcm9092866
[5]
Adnane S, Marino A, Leucci E.LncRNAs in human cancers: signal from noise[J]. Trends Cell Biol, 2022,32(7):565-573. DOI: 10.1016/j.tcb.2022.01.006
[6]
Zhang L, Meng X, Zhu XW, et al.Long non-coding RNAs in oral squamous cell carcinoma: biologic function, mechanisms and clinical implications[J]. Mol Cancer, 2019,18(1):102. DOI: 10.1186/s12943-019-1021-3
[7]
Zhu W, Wang J, Liu X, et al.lncRNA CYTOR promotes aberrant glycolysis and mitochondrial respiration via HNRNPC-mediated ZEB1 stabilization in oral squamous cell carcinoma[J]. Cell Death Dis, 2022,13(8):703. DOI: 10.1038/s41419-022-05157-1
[8]
Shi L, Yang Y, Li M, et al.LncRNA IFITM4P promotes immune escape by up-regulating PD-L1 via dual mechanism in oral carcinogenesis[J]. Mol Ther, 2022,30(4):1564-1577. DOI: 10.1016/j.ymthe.2022.01.003
[9]
Gao C, Guo C, Liu K, et al.LncRNA HOTAIR promotes proliferation and migration of OSCC cells via targeting miR-126[J]. Minerva Gastroenterol (Torino), 2022,68(2):245-247. DOI: 10.23736/S2724-5985.21.02993-4
[10]
Jiang L, Zhou B, Fu D, et al.lncRNA TUG1 promotes the development of oral squamous cell carcinoma by regulating the MAPK signaling pathway by sponging miR-593-3p[J]. Cell Cycle, 2022,21(17):1856-1866. DOI: 10.1080/15384101.2022.2074624
[11]
Tang H, Wu Z, Zhang Y, et al.Identification and function analysis of a five-long noncoding RNA prognostic signature for endometrial cancer patients[J]. DNA Cell Biol, 2019,38(12):1480-1498. DOI: 10.1089/dna.2019.4944
[12]
Huang P, Xu Q, Yan Y, et al.HBx/ERα complex-mediated LINC01352 downregulation promotes HBV-related hepatocellular carcinoma via the miR-135b-APC axis[J]. Oncogene, 2020,39(18):3774-3789. DOI: 10.1038/s41388-020-1254-z
[13]
Zhang C, Zhou D, Wang Z, et al.Risk model and immune signature of m7G-related LncRNA based on lung adenocarcinoma[J]. Front Genet, 2022,13:907754. DOI: 10.3389/fgene.2022.907754
[14]
Szczepanek J, Skorupa M, Tretyn A.MicroRNA as a potential therapeutic molecule in cancer[J]. Cells, 2022,11(6):1008. DOI: 10.3390/cells11061008
[15]
Gong J, Ma L, Peng C, et al.LncRNA MAGI2-AS3 acts as a tumor suppressor that attenuates non-small cell lung cancer progression by targeting the miR-629-5p/TXNIP axis[J]. Ann Transl Med, 2021,9(24):1793. DOI: 10.21037/atm-21-6466
[16]
Yan G, Chang Z, Wang C, et al.LncRNA ILF3-AS1 promotes cell migration, invasion and EMT process in hepatocellular carcinoma via the miR-628-5p/MEIS2 axis to activate the Notch pathway[J]. Dig Liver Dis, 2022,54(1):125-135. DOI: 10.1016/j.dld.2021.04.036
[17]
邓莹莹,吕永利,夏天,等.鞣花酸通过调控miR-1254表达抑制口腔鳞癌HSC3细胞增殖、迁移和侵袭的机制[J].河北医药,2023,45(6):810-813,819. DOI: 10.3969/j.issn.1002-7386.2023.06.002.Deng YY, Lyu YL, Xia T, et al.Ellagic acid inhibits the proliferation, migration and invasion of the oral squamous cell carcinoma cell line HSC3 by regulating miR-1254[J]. Hebei Medical Journal, 2023,45(6):810-813,819. DOI: 10.3969/j.issn.1002-7386.2023.06.002
[18]
Jang TH, Huang WC, Tung SL, et al.MicroRNA-485-5p targets keratin 17 to regulate oral cancer stemness and chemoresistance via the integrin/FAK/Src/ERK/β-catenin pathway[J]. J Biomed Sci, 2022,29(1):42. DOI: 10.1186/s12929-022-00824-z
[19]
Piao Y, Jung SN, Lim MA, et al.A circulating microRNA panel as a novel dynamic monitor for oral squamous cell carcinoma[J]. Sci Rep, 2023,13(1):2000. DOI: 10.1038/s41598-023-28550-y
[20]
Jiang M, Li B.STAT3 and Its Targeting inhibitors in oral squamous cell carcinoma[J]. Cells, 2022,11(19):3131. DOI: 10.3390/cells11193131
[21]
Kaminagakura E, Tango RN, Cruz-Perez D, et al.Oral squamous cell carcinoma outcome in adolescent/young adult: systematic review and meta-analysis[J]. Head Neck, 2022,44(2):548-561. DOI: 10.1002/hed.26940
[22]
Zou C, Lv X, Wei H, et al.Long non-coding RNA LINC00472 inhibits oral squamous cell carcinoma via miR-4311/GNG7 axis[J]. Bioengineered, 2022,13(3):6371-6382. DOI: 10.1080/21655979.2022.2040768
[23]
张影,姚曼曼,刘珊珊,等.LncRNA-NKILA靶向miR-3195对口腔癌细胞活性及血管生成的作用机制[J].河北医学,2022,28(10):1591-1597. DOI: 10.3969/j.jssn.1006-6233.2022.10.02.Zhang Y, Yao MM, Liu SS, et al.LncRNA-NKILA targeting miR-3195 mechanism on oral cell cell activity and vascular generation[J]. Hebei Medicine, 2022, 28(10):1591-1597. DOI: 10.3969/j.jssn.1006-6233.2022.10.02
[24]
Singh D, Assaraf YG, Gacche RN.Long non-coding RNA mediated drug resistance in breast cancer[J]. Drug Resist Updat, 2022,63:100851. DOI: 10.1016/j.drup.2022.100851
[25]
Li M, Mao S, Li L, et al.Hypoxia-related LncRNAs signature predicts prognosis and is associated with immune infiltration and progress of head and neck squamous cell carcinoma[J]. Biochem Biophys Rep, 2022,31:101304. DOI: 10.1016/j.bbrep.2022.101304
[26]
Zhang K, Zhang L, Mi Y, et al.A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration[J]. Cancer Sci, 2020,111(11):4041-4050. DOI: 10.1111/cas.14634
[27]
Li Y, Zeng S, Cao L.Mir-629 repressed LATS2 expression and promoted the proliferation of prostate cancer cells[J]. Horm Metab Res, 2023. DOI: 10.1055/a-2065-0954
[28]
Zhang X, Zhao Y, Wang W, et al.Upregulation of circ_0059961 suppresses cholangiocarcinoma development by modulating miR-629-5p/SFRP2 axis[J]. Pathol Res Pract, 2022,234:153901. DOI: 10.1016/j.prp.2022.153901
2023, Vol. 28 
