Abstract：Objective:To explore the effect of lower limb swaddling position on the development of rat hip and the expression of Collagen-Ⅱ, Aggrecan and Matrix Metalloproteittases-13 in the cartilage of rat hip joint. Methods:We randomly divided 64 newborn rats into two different groups with 33 members in the experimental group and 31 members in the control group. We tied up the lower limb of the rat into a lower limb swaddling position with medical adhesive plaster for one week.8 weeks later, we took pelvic photograph of each rat and compared the transverse diameter of both femoral head and acetabulum as well as acetabular depth and femoral head height in different groups. We determinated the expression of Collagen-Ⅱ,aggrecan and MMP-13 both in the cartilage of femoral head and acetabulum in each group through Real-time PCR.Results:Through imaging and hip anatomy gross morphology, the femoral head and acetabular of experimental group displayed the performance of hip dysplasia and joint degeneration. By the 8th week of time, compared with the control group, we detected significant diversity in transverse diameter of both femoral head and acetabulum in the experiment group(P＜0.05). So it was with the obvious diversity in acetabular depth between two groups(P＜0.05). Through Real-time PCR, we observed lower expression of Collagen-Ⅱ and Aggrecan but higher MMP-13 in cartilage of femoral head from experimental group (P＜0.05) compared with the control group. However, we observed up-regulation of Collagen-Ⅱ,MMP-13 and Aggrecan from cartilage of acetabular in the experimental group(P＜0.05).Conclusions:Binding of lower limb swaddling position with plastic belts can induce developmental dysplasia of the hip (DDH) as well as hip joint degeneration. The degeneration of rat hip joint may stem from up-regulation of MMP-13 in cartilage, which secondary suppress the expression of Collagen-Ⅱ and Aggrecan in chondrocyte of hip joint.
王晖，李政，张翔，朱俊峰，沈超，崔一民，郑新峰，谭华平，蒋雷生，陈晓东. 襁褓体位对大鼠髋关节软骨中二型胶原、蛋白聚糖及金属基质蛋白酶-13表达的影响[J]. 中华解剖与临床杂志, 2013, 18(5): 385-389.
WANG Hui,LI Zheng,ZHANG Xiang,ZHU Jun-feng,SHEN Chao,CUI Yi-min,ZHENG Xin-feng,TAN Hua-ping,JIANG Lei-sheng,CHEN Xiao-dong. The Effect of Swaddling Position on the Expression of Collagen-Ⅱ, Aggrecan and Matrix Metalloproteittases 13 in the Cartilage of Rat Hip. Chinese Journal of Anatomy and Clinics, 2013, 18(5): 385-389.
2013, Vol. 18 
