Abstract：Objective To explore the microencapsulated olfactory ensheathing cell (MC-OECs) transplantation on the treatment of neuropathic pain, and to observe the L4-5 spinal cord P2X2, P2X3 receptors expression and effects.Methods The differential adherent Methods were used to culture olfactory ensheathing cells (OECs) from olfactory bulb of SD rats from September 2014 to July 2015. The 9th generations of OECs were identified by immunofluorescence staining, then to produce MC-OECs. Forty-eight healthy SD rats regardless of males and females were randomly divided into control groups, model groups, OECs groups and MC-OECs groups, 12 in each group. Sciatic nerve injury animal models were set up in model groups, OECs groups and MC-OECs groups. The same amount of OECs and MC-OECs 1 mL (2.1×106/mL) with absorbable Mingjiao sponge placed on both sides of the sciatic nerve injury of OECs groups and MC-OECs groups. Each group of SD rat L4-5 spinal cord were taken after 7, 14 days postoperatively. Situ hybridization technique was used to observe the expression of P2X2, P2X3 receptor positive cells in four groups.Results The Results of situ hybridization showed that P2X2, P2X3 receptors expression in the spinal cord grey matter. The nucleus express clearly and Cytoplasm within a small amount of expression. After 7, 14 days, the Model groups, OECS groups, MC-OECS groups and the control groups P2X2, P2X3 receptor positive cells percentage and the average cells absorbance were reduced in turn. And comparison between groups the differences were statistically significant(all P values<0.05).Conclusions The transplantation of microencapsulated olfactory ensheathing cells can reduce the expression levels of P2X2, P2X3 receptors at spinal cord and suppress and relieve neuropathic pain.
张文俊, 杨宝林, 刘怡果, 刘曾旭. 移植微囊化嗅鞘细胞对P2X2、P2X3受体介导神经病理性疼痛影响的实验研究[J]. 中华解剖与临床杂志, 2017, 22(1): 58-63.
Zhang Wenjun, Yang Baolin, Liu Yiguo, Liu Zengxu.. The effect of microencapsulated olfactory ensheathing cell transplantation on P2X2 and P2X3 receptor-mediated neuropathic pain. Chinese Journal of Anatomy and Clinics, 2017, 22(1): 58-63.
Wang ZT, Yu G, Wang HS, et al. Changes in VGLUT2 expression and function in pain-related supraspinal regions correlate with the pathogenesis of neuropathic pain in a mouse spared nerve injury model[J]. Brain Res, 2015, 1624: 515-524. DOI:10.1016/j.brainres.2015.08.010
[3]
Liu ZX, Zhao H, Yang BL, et al. Microencapsulation improves inhibitory effects of transplanted olfactory ensheathing cells on pain after sciatic nerve injury[J]. Neural Regen Res, 2015, 10(8): 1332. DOI:10.4103/1673-5374.162769
[4]
Schestatsky P, Vidor L, Winckler PB, et al. Promising treatments for neuropathic pain[J]. Arq Neuropsiquiatr, 2014, 72(11): 881-888
[5]
Seixas D, Foley P, Palace J, et al. Pain in multiple sclerosis: a systematic review of neuroimaging studies[J]. Neuroimage Clin, 2014, 5: 322-331. DOI:10.1016/j.nicl.2014.06.014
[6]
Vickers ER, Karsten E, Flood J, et al. A preliminary report on stem cell therapy for neuropathic pain in humans[J]. J Pain Res, 2014, 7: 255-263.DOI:10.2147/JPR.S63361
[7]
Wang YC, Xia QJ, Ba YC, et al. Transplantation of olfactory ensheathing cells promotes the recovery of neurological functions in rats with traumatic brain injury associated with downregulation of Bad[J]. Cytotherapy, 2014, 16(7): 1000-1010. DOI:10.1016/j.jcyt.2013.12.009
[8]
Roet KC, Verhaagen J. Understanding the neural repair-promoting properties of olfactory ensheathing cells[J]. Exp Neurol, 2014, 261: 594-609. DOI:10.1016/j.expneurol.2014.05.007
[9]
Mackay-Sim A, Féron F, Cochrane J, et al. Autologous olfactory ensheathing cell transplantation in human paraplegia: a 3-year clinical trial[J]. Brain, 2008, 131(Pt 9): 2376-2386. DOI:10.1093/brain/awn173
[10]
Zheng Z, Liu G, Chen Y, et al. Olfactory ensheathing cell transplantation improves sympathetic skin responses in chronic spinal cord injury[J]. Neural Regen Res, 2013, 8(30): 2849-2855. DOI:10.3969/j.issn.1673-5374.2013.30.007
[11]
Lang BC, Zhang Z, Lv LY, et al. OECs transplantation results in neuropathic pain associated with BDNF regulating ERK activity in rats following cord hemisection[J]. BMC Neurosci, 2013, 14: 80. DOI:10.1186/1471-2202-14-80
Prado FC, Araldi D, Vieira AS, et al. Neuronal P2X3 receptor activation is essential to the hyperalgesia induced by prostaglandins and sympathomimetic amines released during inflammation[J]. Neuropharmacology, 2013, 67: 252-258. DOI:10.1016/j.neuropharm.2012.11.011
[14]
Wang WS, Tu WZ, Cheng RD, et al. Electroacupuncture and A-317491 depress the transmission of pain on primary afferent mediated by the P2X3 receptor in rats with chronic neuropathic pain states[J]. J Neurosci Res, 2014, 92(12): 1703-1713. DOI:10.1002/jnr.23451
[15]
Müller CE. Medicinal chemistry of P2X receptors: allosteric modulators[J]. Curr Med Chem, 2015, 22(7): 929-941. DOI:10.2174/0929867322666141210155610
[16]
Gao Y, Liu H, Deng L, et al. Effect of emodin on neuropathic pain transmission mediated by P2X2/3 receptor of primary sensory neurons[J]. Brain Res Bull, 2011, 84(6): 406-413. DOI:10.1016/j.brainresbull.2011.01.017
[17]
Lin J, Li G, Den X, et al. VEGF and its receptor-2 involved in neuropathic pain transmission mediated by P2X2(/)3 receptor of primary sensory neurons[J]. Brain Res Bull, 2010, 83(5): 284-291. DOI:10.1016/j.brainresbull.2010.08.002
2017, Vol. 22 
