Abstract：Objective This study aimed to investigate the protective effects and underlying mechanism of ischemic preconditioning (IPC)-induced renal progenitor cell (RPC) homing to renal ischemia-reperfusion injury (IRI) in nephron sparing surgery (NSS).Methods Fifty-four male Sprague-Dawley rats aged 2-3 months and weighing 250-300 g were selected. The rats were randomly divided into three groups with a random number table following right-side nephrectomy. Eighteen rats were included in the three groups, namely, sham operation group (Sham group, surgery without vascular clamping), simple NSS group (NSS group, renal artery clamping for 45 min with NSS), and ischemic preconditioning+NSS group (IPC group, pretreated with 15 min ischemia and 10 min reperfusion before NSS). Six rats were taken from each of the three groups, blood and kidney tissue samples were collected at 12, 24, and 72 h after surgery. The rats were then sacrificed through cervical dislocation. The observation items were as follows: (1) Detection of serum creatinine (SCr) and blood urea nitrogen (BUN); (2) Histopathological examination and renal tubular injury score; (3) The effects of IPC on RPCs and the effects of stromal cell-derived factor (SDF-1) and receptor CXC chemokine receptor type 7 (CXCR7) expression levels.Results (1) The SCr and BUN of the IPC [(65.0±10.78), (91.5±15.12), (52.6±11.68)μmol/L and (14.78±2.77), (18.31±4.99), (9.41±2.73) mmol/L] and the NSS groups [(80.5±12.63), (116.9±14.32), (83.7±11.43) μmol/L and (18.58±4.18), (28.86±5.64), (19.49±3.83) mmol/L] were higher than in the Sham group [(41.5±7.36), (39.7±7.55), (42.7±7.15) μmol/L and (7.72±1.75), (7.40±1.98), (6.83±2.09) mmol/L] at 12, 24, and 72 h after surgery, and the IPC group was lower than the NSS group. Although no statistically significant difference was found between the IPC and Sham groups at 72 h after surgery (P>0.05), the difference was statistically significant (all P values<0.05).(2)At 12, 24, and 72 h after surgery, the renal tubular injury scores in the IPC and NSS groups were higher than those in the Sham group, and the renal tubular injury scores in the IPC group were lower than those in the NSS group at 12 and 24 h. The difference was statistically significant (all P values<0.05). (3) At 24 h after surgery, the number of RPCs in the nephron and the expression levels of SDF-1 and CXCR7 in the kidney significantly increased. The difference was statistically significant (all P values<0.05).Conclusions The results suggest that IPC can initiate the mobilization and homing of RPCs to attenuate renal IRI after NSS. SDF-1/CXCR7 may play a crucial role in this process.
刘敬宇, 辛慧, 周昌成, 吴然, 贾瑞鹏. 缺血预适应对大鼠保留肾单位手术中缺血再灌注损伤的保护作用及其机制研究[J]. 中华解剖与临床杂志, 2020, 25(5): 548-554.
Liu Jingyu, Xin Hui, Zhou Changcheng, Wu Ran, Jia Ruipeng. Protective effects and mechanism of ischemic preconditioning in ischemia-reperfusion injury after nephron sparing surgery in rats. Chinese Journal of Anatomy and Clinics, 2020, 25(5): 548-554.
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2020, Vol. 25 
