Abstract：Objective To investigate the influences of glucagon-like peptide-2(GLP-2)on intestinal mucosal immune barrier in mice with sever acute pancreatitis(SAP).Methods All healthy female BALB/C mice were divided into normal control, SAP and GLP-2 group by random allocation. SAP mice model was established by intraperitoneal injections with large dose L-Arg. The changes of CD3+, CD4+, CD8+ lymphocyte and IgA λ chain of intestinal mucous membrane and amylase of blood and ascites were observed. The same method was used in GLP-2 group to observe the changes of the above indices.Results The blood and ascites amylase value in SAP groups were (1 260.93±58.99)U/L and (389.33±30.31)U/L respectively, were significantly higher than those in NC group and GLP-2 treatment group (all P values<0.01). Expression of SAP in intestinal mucosa of mice CD3+, CD4+, CD8+ lymphocytes and IgA λ chain were (19.57±6.56)/HP,(5.49±2.97)/HP,(2.96±1.22)/HP and (6.20±3.22)/HP respectively, were obviously higher than those of the NC group and the SAP group(all P values<0.05). Compared with the NC group, expression of mice CD4+ lymphocytes and IgAλ chain of GLP-2 treatment group had no statistical significance (all P values>0.05).Conclusions GLP-2 can increase the expression of CD3+, CD4+, CD8+ lymphocytes and IgA λ chain in intestinal mucosa of SAP mice, and has the protective effect on intestinal immune barrier.
孙凡华，许建国，张宗兵，孔令尚，汪华学，刘牧林，程兴望，何先弟. 胰高血糖素样肽-2对急性胰腺炎小鼠肠黏膜免疫屏障的影响[J]. 中华解剖与临床杂志, 2014, 19(4): 316-319.
Sun Fanhua , Xu Jianguo, Zhang Zongbing, Kong Lingshang, Wang Huaxue, Liu Mulin, Cheng Xingwang, He Xiandi. Effect of glucagon-like peptide-2 on intestinal mucosal immune barrier in mice with acute pancreatitis. Chinese Journal of Anatomy and Clinics, 2014, 19(4): 316-319.
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2014, Vol. 19 
