Abstract：Objective To explore the relationship between the serum interleukin-6(IL-6) levels, cecal ligation, perforation(CLP) , colon ascendens stent peritonitisc(CASP) and the survival rate of mice during early stage.Methods Sixty male BALB/c mice were divided into three groups by using random digits table(20 mice in each group), which were Sham group, CLP group and CASP group. In the CLP group, a midline laparotomy was performed and the cecum was fully exposed with adjoining intestine after complete anesthesia. The cecum was tightly ligated at its base and punctured twice by an 18 G needle. The cecum was then gently squeezed to extrude a small amount of stool from the perforation sites. CASP group was similar to the preoperation preparation and the "open" method of CLP group. After exposure of the ascending colon, the prepared catheter was stitched through the antimesenteric wall into the lumen of the ascending colon and was then fixed with two stitches. Consecutively, the inner needle of the stent was removed and the stent was cut as the prepared site. To ensure the proper intraluminal positioning of the stent, stool was milked from the cecum into the ascending colon and the stent until a small drop of stool appeared. The survival rates of several time points after surgery were observed in each group. Six hours after the surgery, the ELISA was developed for detecting murine serum IL-6 in these three groups and the IL-6 levels of living and dead ones in both CLP and CASP group were compared.Results The 72 h survival rates of Sham, CLP and CASP group were 100.0%(20/20), 45.0%(9/20) and 10.0%(2/20) respectively , the differences was statistically significant (χ2=32.970, P＜0.01). Compared in pairs, the differences between all three groups were significant (all P values＜0.05). Differences in survival were assessed by using Log-rank test, and which had statistical difference (χ2=34.030, P＜0.05). On the 6th hour postoperatively, the IL-6 levels of Sham group, CLP group and CASP group were (36.62±10.30)ng/L, (2 443.47±970.50)ng/L, (4 057.93±827.41)ng/L, respectively, with statistical significance (Hc=29.270, P＜0.01). The serum IL-6 levels, of the living mice[(1 348.80±276.25)ng/L, 2 100.00 ng/L] were lower than the dead ones[(3 157.29±330.94)ng/L, (4 275.48±512.71)ng/L], 6 h after the surgery, in both CLP and CASP group (tCLP=13.071, P<0.01).Conlusions With certain mortalities, these two animal models closely mimic the clinical course of intra-abdominal sepsis. The CLP model mainly emphasizes to stimulate the local peritoneal abscess while the CASP model is similar to diffuse peritonitis. The group with lower serum IL-6 levels 6 h after surgery has higher survival rate 72 h after the operation.
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Bi Zhifei, Lu Li, Hu Xiaoqin, Long Nyu, Yang Guijiao.. Relationship between interleukin-6 and the early survival rate of mice in two septic models. Chinese Journal of Anatomy and Clinics, 2015, 20(2): 142-145.
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