Abstract：Objective To investigate the effects of crystal solution, colloidal solution and crystal glue solution in restrictive resuscitation on interleukin(IL)-4 and IL-10 in rats of hemorrhagic shock.Methods Twenty-four healthy SD rats were randomly divided into four groups: one normal control group and three experimental groups, the latter contained sodium Lactated Ringer′s group (crystal group), Hydroxyethyl Starch group (colloidal group), sodium Lactated Ringer′s and Hydroxyethyl Starch group (crystal glue group). The hemorrhagic shock models were established in the three experimental groups by bleeding through the femoral artery. Peripheral blood of rats in each experimental group was collected for five times: before shock (T0), after 30 minutes of keeping mean arterial pressure(MAP) in 40 mmHg (T1), after 30 minutes of keeping MAP in 60 mmHg(T2), after 30 minutes of keeping MAP in 80 mmHg (T3), and at 24h after successful resuscitation(T4). The blood of rats in normal control group was only collected at the same time when T0 and T4 came. ELISA method was used to measure IL-4 and IL-10 content in plasma.Results There were no significant differences in the age, weight and MAP before experiment among all groups (all P values＞0.05). Before shock (at T0) , the contents of IL-4 and IL-10 in plasma of each group rats had no statistical significance(all P values＞0.05). When the shock began (at T1 and T2), the contents of IL-4 and IL-10 in plasma of each experimental group rats had no statistical significance(all P values＞0.05). In the process of restrictive resuscitation, the contents of IL-4 and IL-10 in crystal group and colloidal group were decreasing. The contents of IL-4 and IL-10 in crystal glue group were higher than that of the crystal group or colloidal group at T3 and T4. The contents of IL-4 and IL-10 in normal control group were higher than that of the crystal group or colloidal group at T4, the difference had statistical significance (all P values＜0.01).Conlusions In the process of restrictive resuscitation, crystal glue solution can better accelerate the release of anti-inflammatory mediators. Crystal glue solution may be a relatively better restrictive resuscitation liquid.
孟涛, 王振杰, 房漫军, 杜召辉. 三种液体限制性复苏对失血性休克大鼠血浆白细胞介素-4、-10的影响[J]. 中华解剖与临床杂志, 2015, 20(2): 146-149.
Meng Tao, Wang Zhenjie, Fang Manjun, Du Zhaohui. Effects of three fluids during restrictive resuscitation on interleukin-4 and interleukin-10 in rats of hemorrhagic shock. Chinese Journal of Anatomy and Clinics, 2015, 20(2): 146-149.
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