Mapping fetal specimens brain development of 10-weeks gestational age with 9.4 T magnetic resonance imaging and histopathological features
Gao Yue1, He Xue2, Lin Xiangtao3, Zhou Chenxi1, Liu Shuwei3, Liu Weiwei1, Wang Ximing4, Zhang Zhonghe4
1Department of Medical Imaging,Maternity and Child Care Center of Dezhou, Dezhou 253000, China; 2Department of Imaging,Heze Peony People's Hospital, Heze 274000, China; 3Research Center for Sectional and Imaging Anatomy, Shandong University Cheeloo College of Medicine, Jinan 250012, China; 4Department of Medical Imaging, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China
摘要目的 探讨10孕周胎儿标本脑发育结构的9.4 T超高场强MR影像及其与组织病理学图像的对应关系。方法 纳入山东大学齐鲁医学院断层影像解剖学研究中心的6具10孕周发育正常的自发性流产胎儿标本为研究对象。使用9.4 T MR仪行颅脑T2加权像（T2WI）扫描并三维重建,观察脑组织结构及形态;脑组织切片染色获取组织病理学图像,对比MRI与组织病理学两种检查方法对脑层状结构、侧脑室、小脑等的显示情况。结果 10孕周胎儿脑层状结构在9.4 T MRI上显示为4层,组织病理学图像则显示为6层,其中侧脑室层、皮质层、边缘层在MRI与组织病理学图像可清晰显示;MRI所显示的混合的室周层、室下层、中间层、皮质下层于组织病理学图像上显示为室周层,混合的室下层、中间层,皮质下层。侧脑室内脉络丛MRI显示为3层,边缘呈低信号,中间呈稍低信号,中心呈更低信号;组织病理学图像亦表现为3层,边缘为上皮细胞,中间为结缔纤维基质,中心为血管组织：MRI形态学特点与病理图像对应。小脑表面光滑,MRI呈低信号,无法具体显示各层;组织病理学图像各层结构显示清晰,由内至外为中间层、分子层、颗粒前体细胞层。脑组织结构经MRI三维重建可直观显示其形态、位置及毗邻关系。10孕周胎儿脑体积较小,脉络丛位于侧脑室内,侧脑室外为层状结构及大脑半球,小脑匐于中脑后方,局部与中脑相连。脉络丛占侧脑室体积的40.4%±1.3%,侧脑室占大脑半球体积的46.1%±4.7%。结论 10孕周胎脑尚处于发育的早期阶段,其9.4 T MRI显示与组织病理学图像显示有很好的一致性,而三维重建可以更好地显示10孕周胎脑结构的特点,为临床医师早期识别胎儿发育相关性疾病提供参考。
Abstract：Objective This study aimed to delineate the fetal specimens brain structures at 10-weeks gestational age (GA) using 9.4 T ultra-high field magnetic resonance imaging (MRI) and analyze its relationship with corresponding histopathological sections. Methods Six fetal specimens at 10 weeks GA with normal brain development were included in this study. The specimens were obtained because of spontaneous abortion and provided by the Research Center for Sectional and Imaging Anatomy of Shandong University Cheeloo College of Medicine. They were scanned by using 9.4 T MRI, and then sectioned and dyed to obtain the corresponding histopathological sections. The fetal brain structures were comparatively observed on the MRIs and histopathological sections, and 3D reconstruction models were obtained. Results Four zones of the laminar organization were found in 9.4 T MRIs, whereas six zones were delineated in the corresponding histopathological sections. The ventricular zone, cortical plate, and marginal zone were delineated clearly on MRIs and histopathological sections. The mixed periventricular zone, subventricular zone, intermediate zone, and subplate zone were found on MRIs, whereas the periventricular zone, mixed subventricular zone, intermediate zone, and subplate zone were delineated on histopathological sections. Three zones of the choroid plexus in the lateral ventricles were observed on MRIs, with low signal intensity around the edges, slightly lower signal in the middle, and considerably lower signal intensity in the center, and on histopathological sections with epithelial cells on the edge, associated fiber matrix in the middle, and vascular tissue in the center. Good consistencies were also observed between the MRIs and histopathological sections. The surface of the cerebellum was smooth with low signal on MRIs, and each zone could not be found on histopathological sections, including the intermediate zone, molecular zone, and granular precursor cell zone from the inside to the outside. MRI 3D reconstruction models could directly delineate the shape, position, and adjacency of fetal brain structures. The fetal brain volume at 10 weeks GA was small. The choroid plexus was located in the lateral ventricles, while the laminar organization and cerebral hemisphere were located outside of the lateral ventricles. The cerebellum was crawled behind the midbrain and locally connected with the midbrain. The proportion of the lateral ventricles and choroid plexus was large in the brain, with the choroid plexus accounting for 40.4%±1.3% of the lateral ventricles and the lateral ventricles accounting for 46.1%±4.7% of the cerebral hemisphere. Conclusion The fetal brain at 10 weeks GA is still at an early stage of development. In addition, good consistence is observed between the 9.4 T MRI and histopathological sections in delineating the fetal brain structures. The combination of the 9.4 T MRI and 3D reconstruction can better reveal the characteristics of the fetal brain structure at 10 weeks GA, which can provide a reference for clinicians to identify early diseases related to fetal development.
杲悦, 何雪, 林祥涛, 周晨曦, 刘树伟, 刘玮玮, 王锡明, 张忠和. 10孕周胎儿标本脑发育结构9.4 T MRI表现与组织病理学特征的对照研究[J]. 中华解剖与临床杂志, 2023, 28(11): 697-702.
Gao Yue, He Xue, Lin Xiangtao, Zhou Chenxi, Liu Shuwei, Liu Weiwei, Wang Ximing, Zhang Zhonghe. Mapping fetal specimens brain development of 10-weeks gestational age with 9.4 T magnetic resonance imaging and histopathological features. Chinese Journal of Anatomy and Clinics, 2023, 28(11): 697-702.
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2023, Vol. 28 
