Establishment of iliac vein stenosis in a pig model
Mao Youjun1, Li Xiaoqiang2, Chen Xiaoyu1, Zhang Weigang1, Tao Yinyu1, Zhuo Huawei1, Sun Yang1, Li Chenglong3, Wang Jing4, Yin Yin4
1Department of Vascular Surgery, Changzhou No. 2 People's Hospital Affiliated to Nanjing Medical University, Changzhou 213000, China; 2Department of Vascular Surgery, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing 210008, China; 3Department of Vascular Surgery, the Second Affiliated Hospital, Soochow University, Suzhou 215004, China; 4Center of Animal Experiment, Soochow University, Suzhou 215123, China
Abstract：Objective To explore the establishment of an animal model of iliac vein stenosis.Methods A total of 12 experimental pigs were randomly divided into the experimental and control groups, with 6 pigs in each group. In the experimental group, the left iliac vein was attached to a quartz tube, and then areas at 1-2 cm and 3-5 cm below the left iliac vein into the inferior vena cava were ligated, and the quartz tube was removed to establish the iliac vein stenosis animal model. The animals in the control group did not undergo operation. The diameter of the left iliac vein was measured via intravenous ultrasound at the location wherein the left iliac vein flowed into the inferior vena cava before operation and on the 30th day after operation in the experimental group. Iliac vein angiography was also performed in the experimental group on the 30th day after operation to observe the patency of iliac vein. After examination, the two groups of experimental animals were killed, and the “human-type” blood vessels from the right iliac vein to 2 cm near the heart and from the left iliac vein to the lower part of the inferior vena cava were obtained to observe pathological changes in the vein wall. The intimal thickness of the left iliac vein in the experimental group and the corresponding part in the control group was measured using Image Pro Plus image processing software. The diameter of the left iliac vein stenosis rate in the experimental group was more than 30%, which was used as the standard for model preparation. The difference in the diameter of the left iliac vein before and after operation was observed and compared by obtaining the difference in the intimal thickness and the histopathological changes of the left iliac vein between the two groups.Results In the experimental group, the diameter of the left iliac vein was 7.28-8.04 (7.53±0.28) mm before operation and 3.72-5.02 (4.39±0.48) mm on the 30th day after operation. The difference in the diameter of the left iliac vein before and after the operation was 2.32-3.88 (3.14±0.57) mm; and the narrowing rate of the diameter of the left iliac vein was 31.6%-51.1% (41.57%±6.85%). All the models were successfully established, and the diameter difference was statistically significant (t=13.575, P<0.05). The right iliac veins was unobstructed, and the left iliac vein was evidently narrowed on the 30th day after operation. The histopathological examination of the vein wall in the experimental and control groups showed that the control group exhibited no abnormality; by contrast, the experimental group suffered from hyperplasia and stenosis. The intimal thickness of the left iliac vein in the experimental group was (209.82±26.26) μm, which was significantly greater than that in the control group (37.67±6.84 μm), and their difference was statistically significant (t=15.539, P<0.05).Conclusions The method performed on the experimental group can successfully establish an iliac vein stenosis animal model and exhibits the advantages of simplicity, stability, and reliability.
毛由军, 李晓强, 陈小宇, 张卫刚, 陶银煜, 卓华威, 孙洋, 李承龙, 王婧, 殷音. 猪髂静脉狭窄模型的建立[J]. 中华解剖与临床杂志, 2020, 25(5): 555-559.
Mao Youjun, Li Xiaoqiang, Chen Xiaoyu, Zhang Weigang, Tao Yinyu, Zhuo Huawei, Sun Yang, Li Chenglong, Wang Jing, Yin Yin. Establishment of iliac vein stenosis in a pig model. Chinese Journal of Anatomy and Clinics, 2020, 25(5): 555-559.
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2020, Vol. 25 
