Abstract：Objective To establish radial bone defect model on different month-aged rabbits and to explore bone defect size.Methods Forty healthy male New Zealand rabbits aged three months and six months were included in this study. Based on their age, the rabbits were divided into group A (3 months) and group B (6 months). Before surgery, all the forelimbs were randomly divided into 2 sub-groups by digital table method with a total of 20 sides in each group. The bone defect size of group A1 and B1 was 15 mm, and 20 mm for group A2 and B2. At 4-week intervals postoperatively, X-ray was taken, and the healing of the bone defect was quantitatively evaluated with Lane-Sandhu scores. The rabbits were sacrificed 12 weeks postoperatively and the forearms were harvested for gross observation and histological analysis.Results All the rabbits survived after operation of bone defect model. The X-ray images showed that the bone defect of group A1 almost healed 8 weeks postoperatively. Perfectly plastic bone as normal radius was observed in group A1, but none of the rest three groups were completely repaired 12 weeks postoperatively. Small amount of callus was noticed at both ends and the ulnar side of radius with closed marrow cavity Lane-Sandhu score comparison of intra-group showed that the score of group A1 was higher than that of group A2 with significant difference (all P values<0.05). However, there was no significant difference between group B1 and B2 (all P values>0.05). Inter-group comparison of sub-groups with same defect size showed that the scores of group A1 and A2 were higher than those of group B1 and B2 with significant difference (all P values<0.05) at each time point postoperatively. Continuous bridged callus with a smooth surface were observed in the radii in group A1. However, both ends were closed in other groups, and the defect areas were filled with fibrous tissue. Histological analysis showed that the bone defect in group A1 had been repaired completely, regular arrangement of osteone was also observed. However, the fibrous tissue was seen in the defect area in group A2, B1 and B2.Conclusions The rabbits' age and bone defect size had significant effects on healing of bone defects. To establish an appropriate rabbit radial bone defect model, we can choose 20 mm for juvenile(3 months) rabbits and 15 mm for adult(6 months) rabbits.
芮敏, 郑欣, 李成宇, 张星晨, 孙少松, 姚圣城, 庞勇, 郭开今. 幼龄兔及成年兔桡骨骨缺损模型中骨缺损大小的对比研究[J]. 中华解剖与临床杂志, 2017, 22(5): 402-406.
Rui Min, Zheng Xin, Li Chengyu, Zhang Xingchen, Sun Shaosong, Yao Shengcheng, Pang Yong, Guo Kaijin.. Comparative study on bone defect size in the radial bone defect model of juvenile rabbits and adult rabbits. Chinese Journal of Anatomy and Clinics, 2017, 22(5): 402-406.
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