Abstract：Objective To detect the distribution and frequency of mobilized endothelial progenitor cells (EPCs) in renal cell carcinoma(RCC) in mice, To explore the role of EPCs in neovascularization of RCC.Methods Male BALB/c nude mice were randomly divided into three groups: Rcc group(Group A, n=35); Sham group(Group B, n=35) and Normal group(Group C, n=6). Mice of three groups were killed respectively at days 21, 28,35,42,49 after RCC group implant ACHN cell line, samples were harvested. The distribution and frequency of EPCs, MVD and tissue mRNA (VEGF, SDF-1, FLK and CXCR4) in NT of Normal group, ST of Sham group, AT and TT of RCC group were evaluated.Results FCM showed that EPCs levels in AT were gradually increased after 28 days, and significantly higher than those in TT, NT and ST (P<0.05). EPCs levels in TT were first increased then gradually decreased but were significantly elevated compared with those in NT (P<0.05). Immunofluorescence showed that EPCs were mainly distributed in the AT. Immunohistochemistry showed MVD in AT was significantly higher than that in TT and NT, but MVD in TT was lower than NT (P<0.05). The mRNA expression of VEGF, FLK, SDF-1 and CXCR4 in AT was significantly increased compared with the TT and NT (P<0.05).Coclusions EPCs promotes the progress of RCC, involving the promotion on neovascularization through releasing angiogenic factors and incorporated into vascular endothelium.
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2014, Vol. 19 
