Connections between the neurokinin 1 receptor-like immunoreactive neurons and the γ-aminobutyric acid- or substance P-positive axonal terminals in locus coeruleus of the rat
Qi Jian, Xu Yan, Zhao Tingbao
Department of Spinal Cord Injury and Rehabilitation, the General Hospital of Jinan Military Command, Jinan 250031, China
Abstract:Objective To investigate the connections between neurokinin 1 (NK1) receptor-like immunoreactive (LI) neurons and -aminobutyric acid- (GABA), substance P- (SP) positive axonal terminals in Locus Coeruleus (LC) of the rat.Methods Immunofluorecent histochemical double-staining combining double-staining of electron microscope technique was used to observe the connections between NK1 receptor-LI neurons and GABA- or SP-positive axonal terminals in 10 male SD rats (250-300 g).Results Under laser confocal microscopy, some of these GABA-, SP-LI axon terminals were observed to make close appositions with NK1 receptor-LI neuronal cell bodies and dendritic processes. Under electron microscope, some of these GABA-LI axon terminals by diaminobenzidine(DAB) were observed principally to make symmetric synapses with NK1 receptor-LI neuronal cell bodies and dendritic processes labeled with nano-gold particles. Some of these SP-LI axon terminals labeled by DAB were observed principally to make asymmetric synapses with NK1 receptor-LI neuronal cell bodies and dendritic processes labeled with nano-gold particles.Conclusions GABA-, SP-LI axon terminals were observed to make close appositions with NK1 receptor-LI neuronal cell bodies and dendritic processes within LC. The NK1 receptor-LI neurons within LC are regulated by the GABA or SP-positive axonal terminals.
祁健,徐岩,赵廷宝. 大鼠蓝斑核内神经激肽1受体阳性神经元与γ-氨基丁酸和 P物质样阳性终末的联系[J]. 中华解剖与临床杂志, 2016, 21(1): 53-56.
Qi Jian, Xu Yan, Zhao Tingbao. Connections between the neurokinin 1 receptor-like immunoreactive neurons and the γ-aminobutyric acid- or substance P-positive axonal terminals in locus coeruleus of the rat. Chinese Journal of Anatomy and Clinics, 2016, 21(1): 53-56.
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