Abstract：Objective Toexplore the shape and functional changes of the proprioceptors in the rabbit anterior cruciate ligament(ACL) caused by the ACL injure in the different parts.Methods Twenty eight New Zealand white rabbits were selected and divided into three experimental groups(12 in group A, 12 in group B and 4 in group C). Group A was partly injured medial ACL model. Group B was partly injured ACL model whose injured location was nearly one-third in tibia. Group C had no surgery. The two groups(group A and B) were examined with somatosensory evoked potential(SEPs) and electromyogram(EMG) and gold chloride staining method to evaluate the shape and functional changes of the nervous structure at 2, 4, 6 months post-operation(four rabbits were sacrificed at each time point).Results The SEPs and EMG′s incubation period and wave amplitude degraded gradually with the prolongation of observing time, and the difference of incubation period and wave amplitude between the two groups(group A and B) and the control was statistically significant (all P values＜0.05) . Also the difference of incubation period and wave amplitude between the group A and the group B was not statistically significant (all P values＞0.05). The number of the proprioceptors in the group A and B gradually reduced with the prolongation of observing time (The two groups were compared with the control, all P values＜0.05),and the morphogenesis of the proprioceptors changed, including the volume of proprioceptors diminuted and part structure depleted. Yet there was no statistical changes between group A and B(all P values＞0.05).Conclusions The proprioceptor number is decreased and the number of abnormal proprioceptor is increased after ACL being injured, and the function also weakened languishingly with the prolongation of observing time. But it does not have a statistically significant in the different injured part. The mechanism may be related to the blood vessels in ligament has a little influence on the overall supply of blood.
忻慰, 赵辉, 陈宜, 周义钦, 王波, 吴海山. 兔前交叉韧带不同部位损伤对韧带内本体感受器的影响[J]. 中华解剖与临床杂志, 2015, 20(1): 67-71.
Xin Wei, Zhao Hui, Chen Yi, Zhou Yiqin, Wang Bo, Wu Haishan. Experimental study of proprioceptor′s change caused by injury of different parts of anterior cruciate ligament in rabbits. Chinese Journal of Anatomy and Clinics, 2015, 20(1): 67-71.
Grigg P, Hoffmann AH. Properties of Ruffini afferents revealed by stress analysis of isolated sections of cat knee capsule[J]. Neurophysiol, 1982, 47(1): 41-54.
[2]
Fridèn T, Roberts D, Ztterstrm R, et al. Proprioceptive defects after an anterior cruciate ligament rupture: the relation to associated anatomical lesions and subjective knee function[J]. Knee Surg Sports Traumatol Arthrosc, 1999, 7(4): 226-231.
[3]
Barrett DS. Proprioception and function after anterior cruciate reconstruction[J]. J Bone Joint Surg Br, 1991, 73(5): 833-837.
[4]
Zahm H. Surigical treatment of rupture of the crucial ligament in dogs with the use of synthetic material[J]. Berl Munch Tierarztl Wochenschr, 1966, 79(1): 1-4.
[5]
Whiteside LA, Sweeney RE Jr. Nutrient pathways of the cruciate ligament. An experimental study using hydrogen wash-out technique[J]. J Bone Joint Surg Am, 1980, 62(7): 1176-1180.
[6]
Freeman MA, Wyke B. The innervation of the knee joint. An anatomical and histological study in the cat[J]. J Anat, 1967, 101(Pt 3): 505-532.
[7]
Arockiaraj J, Korula RJ, Oommen AT, et al. Proprioceptive changes in the contralateral knee joint following anterior cruciate injury[J]. Bone Joint J, 2013, 95-B(2): 188-191.
[8]
Schults RA, Miller DC, Kerr CS, et al. Mehanoreeeptorsin human cmciate ligaments. A histdogical study[J]. J Bone Joint Surg, 1984, 66(7): 1072-1076.
[9]
Denti M, Menteleone M, Berardi A, et al. Anterior cruciate ligament mechanoreceptors: histologic studies on lesions and reconstion[J]. Clin Orthop, 1994, (308): 29-32.
[10]
Adachi N, Ochi M, Uchio Y, et al. Mechanoreeptors in the anteri or cruciate ligament contribute to the joint position sense[J]. Acta Orthop Scand, 2002, 73(3): 330-334.
[11]
Corrigan JP, Cashman WF, Brady MP. Proprioception in the cruciate deficient knee[J]. J Bone Joint Surg Br, 1992, 74(2): 247-250.
[12]
Barrack RL, Skinner HB, Buckley SL. Proprioception in the anterior cruciate deficient knee[J]. Am J Sports Med, 1989, (1): 1-6.
[13]
Roberts D, Fridén T, Stomberg A, et al. Bilateral proprioceptive defects in patients with a unilateral anterior cruciate ligament reconstruction: a comparison between patients and healthy individuals[J]. J Orthop Res, 2000, 18(4): 565-71.
[14]
Fridén T, Roberts D, Ztterstrm R, et al. Proprioception in the nearly extended knee:measurements of position and movement in healthy individuals and in symptomatic anterior cruciate ligament injured patients[J]. Knee Surg Sports Traumatol Arthrosc, 1996, 4(4): 217-224.
[15]
Relph N, Herrington L, Tyson S. The effects of ACL injury on knee proprioception: a meta-analysis[J]. Physiotherapy, 2014, 100(3): 187-195.
[16]
Aim A, Gillquist J. Reconstruction of the anterior cruciate ligament by using the medial third of the patellar ligament Treatment and results[J]. Acta Chir Scand, 1974, 140(4): 289-296.
[17]
Amoczky SP, Rubin RM, Marshall JL, et al.Microvasculature of the cruciate ligaments and its response to injury. An experimental study in dogs[J]. J Bone Joint Surg Am, 1979, 61(8): 1221-1229.
[18]
Claney WG Jr, Narechania RG , Rossenbery TD, et al. Anterior and posterior cruciate ligament reconstruction in rhesus monkeys[J]. J Bone Joint Stag, 1981, 63(8): 1270-1284.
[19]
Robinson D, Halperin N, Nero Z. Devaseularization of the anterior cruciate ligament by synovial stripping in rabbits. An experimental model[J]. Acta Orthop Seand, 1992, 63(5): 502-506.
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