Connections between the neurokinin 1 receptor-like immunoreactive neurons and the γ-aminobutyric acid- or substance P-positive axonal terminals in locus coeruleus of the rat
Qi Jian, Xu Yan, Zhao Tingbao
Department of Spinal Cord Injury and Rehabilitation, the General Hospital of Jinan Military Command, Jinan 250031, China
Abstract：Objective To investigate the connections between neurokinin 1 (NK1) receptor-like immunoreactive (LI) neurons and -aminobutyric acid- (GABA), substance P- (SP) positive axonal terminals in Locus Coeruleus (LC) of the rat.Methods Immunofluorecent histochemical double-staining combining double-staining of electron microscope technique was used to observe the connections between NK1 receptor-LI neurons and GABA- or SP-positive axonal terminals in 10 male SD rats (250-300 g).Results Under laser confocal microscopy, some of these GABA-, SP-LI axon terminals were observed to make close appositions with NK1 receptor-LI neuronal cell bodies and dendritic processes. Under electron microscope, some of these GABA-LI axon terminals by diaminobenzidine(DAB) were observed principally to make symmetric synapses with NK1 receptor-LI neuronal cell bodies and dendritic processes labeled with nano-gold particles. Some of these SP-LI axon terminals labeled by DAB were observed principally to make asymmetric synapses with NK1 receptor-LI neuronal cell bodies and dendritic processes labeled with nano-gold particles.Conclusions GABA-, SP-LI axon terminals were observed to make close appositions with NK1 receptor-LI neuronal cell bodies and dendritic processes within LC. The NK1 receptor-LI neurons within LC are regulated by the GABA or SP-positive axonal terminals.
祁健,徐岩,赵廷宝. 大鼠蓝斑核内神经激肽1受体阳性神经元与γ-氨基丁酸和 P物质样阳性终末的联系[J]. 中华解剖与临床杂志, 2016, 21(1): 53-56.
Qi Jian, Xu Yan, Zhao Tingbao. Connections between the neurokinin 1 receptor-like immunoreactive neurons and the γ-aminobutyric acid- or substance P-positive axonal terminals in locus coeruleus of the rat. Chinese Journal of Anatomy and Clinics, 2016, 21(1): 53-56.
Sara SJ. The locus coeruleus and noradrenergic modulation of cognition[J]. Nat Rev Neurosci, 2009, 10(3): 211-223. DOI:10.1038/nrn2573
[2]
Craig AD. Spinal and trigeminal lamina I input to the locus coeruleus anterogradely labeled with Phaseolus vulgaris leucoagglutinin (PHA-L) in the cat and the monkey[J]. Brain Res,1992, 584(1-2): 325-328
[3]
Voisin DL, Guy N, Chalus M, et al. Nociceptive stimulation activates locus coeruleus neurones projecting to the somatosensory thalamus in the rat[J]. J Physiol, 2005, 566(Pt 3): 929-937. DOI:10.1113/jphysiol.2005.086520
[4]
Wenzel J, Kunde D, David E, et al. Chemoarchitecture of the Guinea pig brain (Cavia porcellus.)[J]. J Hirnforsch, 1971, 11(6): 447-466
[5]
Szabadi E. Modulation of physiological reflexes by pain: role of the locus coeruleus[J]. Front Integr Neurosci, 2012, 6(6): 94. DOI:10.3389/fnint.2012.00094
[6]
Nakaya Y, Kaneko T, Shigemoto R, et al. Immunohistochemical localization of substance P receptor in the central nervous system of the adult rat[J]. J Comp Neurol, 1994, 347(2): 249-274. DOI:10.1002/cne.903470208
[7]
Muto Y, Sakai A, Sakamoto A, et al. Activation of NK1 receptors in the locus coeruleus induces analgesia through noradrenergic-mediated descending inhibition in a rat model of neuropathic pain[J]. Br J Pharmacol, 2012, 166(3): 1047-1057. DOI:10.1111/j.1476-5381.2011.01820.x
[8]
Todd AJ, Spike RC. The localization of classical transmitters and neuropeptides within neurons in laminae Ⅰ-Ⅲ of the mammalian spinal dorsal Horn[J]. Prog Neurobiol, 1993, 41(5): 609-645
[9]
Taylor BK. Spinal inhibitory neurotransmission in neuropathic pain[J]. Curr Pain Headache Rep, 2009, 13(3): 208-214
[10]
Jin X, Cui N, Zhong W, et al. GABAergic synaptic inputs of locus coeruleus neurons in wild-type and Mecp2-null mice[J]. Am J Physiol Cell Physiol, 2013, 304(9): C844-C857. DOI:10.1152/ajpcell.00399.2012
[11]
Li JL, Kaneko T, Mizuno N. Synaptic association of dopaminergic axon terminals and neurokinin-1 receptor-expressing intrinsic neurons in the striatum of the rat[J]. Neurosci Lett, 2002, 324(1): 9-12
[12]
Li YQ. Substance P receptor-like immunoreactive neurons in the caudal spinal trigeminal nucleus send axons to the gelatinosusthalamic nucleus in the rat[J]. J Hirnforsch, 1999, 39(3): 277-282
[13]
Li H, Li YQ. Collateral projection of substance P receptor expressing neurons in the medullary dorsal Horn to bilateral parabrachial nuclei of the rat[J]. Brain Res Bull, 2000, 53(2): 163-169
[14]
Todd AJ. Anatomy of primary afferents and projection neurones in the rat spinal dorsal Horn with particular emphasis on substance P and the neurokinin 1 receptor[J]. Exp Physiol, 2002, 87(2): 245-249
[15]
Polgár E, Campbell AD, Macintyre LM, et al. Phosphorylation of ERK in neurokinin 1 receptor-expressing neurons in laminae Ⅲ and IV of the rat spinal dorsal Horn following noxious stimulation[J]. Mol Pain, 2007, 3(3): 4. DOI:10.1186/1744-8069-3-4
[16]
Wang D, Li YQ, Li JL, et al. γ-Aminobutyric acid- and glycine-immunoreactive neurons postsynaptic to substance P-immunoreactive axon terminals in the superficial layers of the rat medullary dorsal Horn[J]. Neurosci Lett, 2000, 288(3): 187-190
[17]
Mackie M, Hughes DI, Maxwell DJ, et al. Distribution and colocalisation of glutamate decarboxylase isoforms in the rat spinal cord[J]. Neuroscience, 2003, 119(2): 461-472
[18]
Wu SX, Wang W, Li H, et al. The synaptic connectivity that underlies the noxious transmission and modulation within the superficial dorsal Horn of the spinal cord[J]. Prog Neurobiol,2010, 91(1): 38-54. DOI:10.1016/j.pneurobio.2010.01.005
[19]
Kato G, Yasaka T, Katafuchi T, et al. Direct GABAergic and glycinergic inhibition of the substantia gelatinosa from the rostral ventromedial medulla revealed by in vivo patch-clamp analysis in rats[J]. J Neurosci, 2006, 26(6): 1787-1794. DOI:10.1523/JNEUROSCI.4856-05.2006.
2016, Vol. 21 
